Short Note On Carnitine Shuttle: Fatty acids are oxidized in mitochondria, and synthesized in the cytoplasm. Long chain fatty acids must form an active intermediate, fatty acyl CoA, before being oxidized inside mitochondria. Catherine How to Analyze Carnitine and Acylcarnitine? Creative Proteomics Blog Mitochondrial control of fuel switching via carnitine biosynthesis Science Carnitine, Acylcarnitines and Beta Oxidation